Follow us at Facebook. Sperm chromatin integrity was studied using the SCSA-assay, with significant differences observed in the degree of fragmentation intensity DFI although this value was consistently low in all animals studied. Furthermore, the presence of MT1 at the cytoplasmic droplet in immature ejaculated stallion spermatozoa found in one sample and epididymal red deer spermatozoa suggests that melatonin may be involved in specific functions during spermatogenesis and sperm maturation, like protecting spermatozoa from oxidative damage, this activity being mediated through these receptors. Epididymal spermatozoa from corpus and cauda of seven cats were cryopreserved and used for IVF. The lynx is protected under Swedish hunting legislation.
After thawing, the semen extender from the first straw was removed and the sperm pellet was resuspended with Tris buffer control.
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The present study was conducted in an attempt to increase the efficiency of the in vitro production of porcine embryos by optimizing the in vitro fertilization IVF protocol for individual males, with regard to the composition of the fertilization medium experiments 1 and 2 and the length of gamete co-incubation time experiment 3. Cryoprotectant toxicity has been claimed to be the single most limiting factor for the success of cryopreservation. Although sperm suspensions of equivalent quality were produced using Androcoll-E in small and large tubes, the sperm yield was much lower in the latter P less than 0. It is generally recommended that female cats not intended for planned breeding are spayed to reduce the population of feral cats and also because spaying is beneficial for the long-term health of the individual. At the transition between regions 4 and 5, located between the caput and corpus epididymides, histological characteristics change from being that of a maturation function to being typical of a storage function. The ability of feline spermatozoa from caput, corpus and cauda regions of epididymis to undergo capacitation and acrosome reaction, which are required before spermatozoa are able to fertilize oocytes, were investigated. However, motility of spermatozoa from both regions was affected by the freezing thawing process with a significant decline in motility after thaw compared with fresh spermatozoa.